DatasheetsPDF.com

S7806 Dataheets PDF



Part Number S7806
Manufacturers ETC
Logo ETC
Description CpG WIZ Prader-Willi/Angelman Amplification Kit
Datasheet S7806 DatasheetS7806 Datasheet (PDF)

CpG WIZ™ p16 Amplification Kit S7800 CpG WIZ™ p15 Amplification Kit S7802 CpG WIZ™ E-cadherin Amplification Kit S7804 CpG WIZ™ Prader-Willi/Angelman Amplification Kit S7806 FOR RESEARCH USE ONLY Not for use in diagnostic procedures USA & Canada Phone: +1(800) 437-7500 • Fax: +1 (909) 676-9209 • Europe +44 (0) 23 8026 2233 Australia +61 3 9839 2000 • Germany +49-6192-207300 • ISO Registered Worldwide www.chemicon.com • [email protected][email protected] This page left blank intention.

  S7806   S7806


Document
CpG WIZ™ p16 Amplification Kit S7800 CpG WIZ™ p15 Amplification Kit S7802 CpG WIZ™ E-cadherin Amplification Kit S7804 CpG WIZ™ Prader-Willi/Angelman Amplification Kit S7806 FOR RESEARCH USE ONLY Not for use in diagnostic procedures USA & Canada Phone: +1(800) 437-7500 • Fax: +1 (909) 676-9209 • Europe +44 (0) 23 8026 2233 Australia +61 3 9839 2000 • Germany +49-6192-207300 • ISO Registered Worldwide www.chemicon.com • [email protected][email protected] This page left blank intentionally. _________________________________________________________ TABLE OF CONTENTS I. INTRODUCTION .............................................................................. 1 Using this Manual ..................................................................................... 1 Background ............................................................................................... 1 Principles of the Technique....................................................................... 2 Fig 1: DNA Treatment with Sodium Bisulfite....................................... 3 II. KIT COMPONENTS ......................................................................... 4 Materials Required But Not Supplied ....................................................... 5 III. PROTOCOLS.................................................................................... 7 CpG WIZ™ p16 (S7800), p15 (S7802) and E-cadherin (S7804) Amplification Kits ................................................. 7 Experimental Design............................................................................ 7 Fig. 2: Specificity of the CpG WIZ™ p16 Amplification Kit .................................................................................. 9 Amplification Protocol....................................................................... 10 CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit ........... 12 Experimental Design.......................................................................... 12 Fig. 3: Specificity of the CpG WIZ™ Prader-Willi/ Angelman Amplification Kit ............................................................... 14 Amplification Protocol....................................................................... 15 IV. DATA ANALYSIS ........................................................................... 18 CpG WIZ™ p16 (S7800), p15 (S7802) and E-cadherin (S7804) Amplification Kits ............................................. 18 CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit ........... 19 V. TROUBLESHOOTING .................................................................... 20 CpG WIZ™ p16 (S7800), p15 (S7802) and E-cadherin (S7804) Amplification Kits .................................................. 20 CpG WIZ™ Prader-Willi/Angelman (S7806) Amplification Kit ........... 21 i VI. APPENDIX ............................................................................ 23 Laboratory Setup and Precautions .......................................................... 23 Related Products...................................................................................... 23 VII. REFERENCES ................................................................................ 24 References Cited in this Manual ............................................................. 24 Disclaimers ............................................................................................ 25 Warranty.................................................................................................. 25 ii I. INTRODUCTION Using This Manual Please read the entire instruction manual prior to using CpG WIZ™ Amplification Kits. Note that in the Procedures and Troubleshooting sections, instructions are separate for the CpG WIZ™ Prader-Willi/ Angelman Amplification Kit (S7806). Should additional questions arise, assistance is available from Chemicon Technical Service at [email protected] or (800) 437-7500. Background Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of several eukaryotic genes (1). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. The exception is extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes (2, 3) such as those associated with Prader-Willi/Angelman Syndrome (4) and genes on the inactive X-chromosome of females (5, 6). Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (7) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (8, 9). E-cadherin, p16, and p15 are examples of genes that exhibit characteristic hypermethylation. Previously developed methods to determine the methylation status of cytosine include digestion with methylation sensitive rest.


S7805PIC S7806 S7806D


@ 2014 :: Datasheetspdf.com :: Semiconductors datasheet search & download site.
(Privacy Policy & Contact)