Cell Signal. XBP-1 Datasheet
|Total Page||2 Pages|
MSD® XBP-1 Kit
For quantitative determination in human, mouse, and rat whole cell lysate samples
Kit Size Catalog #
5 plates K150QKD-2
25 plates K150QKD-4
2. BSA blocked
3. BSA blocked
4. BSA blocked
X-box binding protein 1 (XBP-1) is a basic-region, leucine zipper type transcription factor that belongs to the CREB/ATF family and mediates one
of the three arms of unfolded protein response (UPR) adaptation to endoplasmic reticulum (ER) stressors. The accumulation of unfolded or
misfolded proteins in the ER is a threat to cell survival and results in a condition known as ER-stress.1,2 To overcome this stress, the ER initiates
specific signaling pathways encompassed by the ER stress response.3 Among these are translational attenuation, upregulation of ER chaperone
proteins and proteins that facilitate folding, activation of NFkB signaling, and, as a last resort, the induction of apoptosis.4-6 Under basal conditions,
XBP-1 is ubiquitously expressed as a 33 kDa XBP-1u isoform. The initiation of ER stress activates the endonuclease activity of the transmembrane
kinase and endoribonuclease inositol-requiring enzyme-1 (IRE-1) in an unconventional cleavage and re-ligation reaction producing an active 52
kDa splice-variant form of XBP-1 known as XBP-1s.5 XBP-1s transcriptionally regulates the production of additional ER chaperones and secretory
proteins to maintain the function of the organelle and the health of the cell.6 XBP-1 is involved in the response of many diseases linked to ER stress
(ischemia, neurodegeneration, cancer, diabetes), which makes this an attractive marker for many therapeutic areas. The MSD XBP-1 assay is
available on 96-well, 4-spot plates. This datasheet outlines the performance of the assay.
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Representative results for the XBP-1 Kit are illustrated below. The signal and ratio values provided are examples; individual results will vary
depending upon the samples tested.
This assay was developed using recombinant human XBP-1 protein (data shown below) as well as with the nuclear fraction of rat hepatoma cells
treated with the ER stress-inducing agents, thapsigargin and tunicamycin (data not shown). This assay recognizes both the spliced and non-spliced
XBP-1 isoforms referred to in the introduction. Recombinant protein or nuclear cell lysate fractions were added to MULTI-SPOT®, 4-spot plates
coated with anti-XBP-1 antibody on one of the four spatially distinct electrodes in each well. XBP-1 was detected with anti-XBP-1 antibody
conjugated with MSD SULFO-TAG™.
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Recombinant Human XBP-1 (ng/mL)
Figure 1: Sample data generated with the XBP-1 Kit. Increased signal is observed with the titration of recombinant human XBP-1 protein.
MSD Phosphoprotein Assays
Data for recombinant XBP-1 protein using the XBP-1 Kit are presented below.
XBP-1 Recombinant Protein
150 9 5.7
2571 179 7.0
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The MSD Advantage
Multiplexing: Multiple analytes can be measured in one well using typical sample volumes of 25 µL or less without compromising speed or performance
Large dynamic range: Linear range of up to five logs enables the measurement of native levels of biomarkers in normal and diseased samples without multiple
Minimal background: The stimulation mechanism (electricity) is decoupled from the response (light signal), minimizing matrix interference
Simple protocols: Only labels bound near the electrode surface are excited, enabling assays with fewer washes
Flexibility: Labels are stable, non-radioactive, and conveniently conjugated to biological molecules
1. Kopito RR. Aggresomes, inclusion bodies and protein aggregation. Trends Cell Biol. 2000 Dec;10(12):524-30.
2. Kaufman RJ. Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev. 1999 May 15;13(10):1211-33.
3. Mori K. Tripartite management of unfolded proteins in the endoplasmic reticulum. Cell. 2000 May 26;101(5):451-4.
4. Harding HP, et al. Transcriptional and translational control in the Mammalian unfolded protein response. Annu Rev Cell Dev Biol. 2002;18:575-99.
5. Yoshida H, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001 Dec 28;107(7):881-91.
6. Lee AH, et al. XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response. Mol Cell Biol. 2003 Nov;23(21):7448-59.
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For Research Use Only. Not for use in diagnostic procedures.
|Features||Datasheet pdf MSD® XBP-1 Kit For quantitative determ ination in human, mouse, and rat whole cell lysate samples Alzheimer’s Dise ase BioProcess Cardiac Cell Signaling C linical Immunology Cytokines Growth Fac tors Hypoxia Immunogenicity Inflammatio n Metabolic Oncology Toxicology Vascula r Catalog Numbers XBP-1 Kit Kit Size Catalog # 1 plate K150QKD-1 5 plate s K150QKD-2 25 plates K150QKD-4 1. XB P-1 2. BSA blocked 3. BSA blocked 4. BS A blocked X-box binding protein 1 (XBP- 1) is a basic-region, leucine zipper ty pe transcription factor that belongs to the CREB/ATF family and mediates one o f the three arms of unfolded protein re sponse (UPR) adaptation to endoplasmic reticulum (ER) stressors. The accumulat ion of unfolded or misfolded proteins i n the ER is a threat to cell survival a nd results in a condition known as ER-s tress.1,2 To overcome this stress, the ER initiates specific signaling pathway s encompassed by the ER stress response .3 Among these are translational attenuation, upregulation of ER c.|
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