Cell Signal. XBP-1 Datasheet

XBP-1 Datasheet PDF, Equivalent


Part Number

XBP-1

Description

Cell Signal

Manufacture

MSD

Total Page 2 Pages
PDF Download
Download XBP-1 Datasheet PDF


XBP-1 Datasheet
MSD® XBP-1 Kit
For quantitative determination in human, mouse, and rat whole cell lysate samples
Alzheimer’s Disease
BioProcess
Cardiac
Cell Signaling
Clinical Immunology
Cytokines
Growth Factors
Hypoxia
Immunogenicity
Inflammation
Metabolic
Oncology
Toxicology
Vascular
Catalog Numbers
XBP-1 Kit
Kit Size Catalog #
1 plate
K150QKD-1
5 plates K150QKD-2
25 plates K150QKD-4
1. XBP-1
2. BSA blocked
3. BSA blocked
4. BSA blocked
X-box binding protein 1 (XBP-1) is a basic-region, leucine zipper type transcription factor that belongs to the CREB/ATF family and mediates one
of the three arms of unfolded protein response (UPR) adaptation to endoplasmic reticulum (ER) stressors. The accumulation of unfolded or
misfolded proteins in the ER is a threat to cell survival and results in a condition known as ER-stress.1,2 To overcome this stress, the ER initiates
specific signaling pathways encompassed by the ER stress response.3 Among these are translational attenuation, upregulation of ER chaperone
proteins and proteins that facilitate folding, activation of NFkB signaling, and, as a last resort, the induction of apoptosis.4-6 Under basal conditions,
XBP-1 is ubiquitously expressed as a 33 kDa XBP-1u isoform. The initiation of ER stress activates the endonuclease activity of the transmembrane
kinase and endoribonuclease inositol-requiring enzyme-1 (IRE-1) in an unconventional cleavage and re-ligation reaction producing an active 52
kDa splice-variant form of XBP-1 known as XBP-1s.5 XBP-1s transcriptionally regulates the production of additional ER chaperones and secretory
proteins to maintain the function of the organelle and the health of the cell.6 XBP-1 is involved in the response of many diseases linked to ER stress
(ischemia, neurodegeneration, cancer, diabetes), which makes this an attractive marker for many therapeutic areas. The MSD XBP-1 assay is
available on 96-well, 4-spot plates. This datasheet outlines the performance of the assay.
Ordering Information
MSD Customer Service
Phone: 1-301-947-2085
Fax: 1-301-990-2776
Email: CustomerService@
mesoscale.com
Scientific Support
Phone: 1-301-947-2025
Email: ScientificSupport@
mesoscale.com
Typical Data
Representative results for the XBP-1 Kit are illustrated below. The signal and ratio values provided are examples; individual results will vary
depending upon the samples tested.
This assay was developed using recombinant human XBP-1 protein (data shown below) as well as with the nuclear fraction of rat hepatoma cells
treated with the ER stress-inducing agents, thapsigargin and tunicamycin (data not shown). This assay recognizes both the spliced and non-spliced
XBP-1 isoforms referred to in the introduction. Recombinant protein or nuclear cell lysate fractions were added to MULTI-SPOT®, 4-spot plates
coated with anti-XBP-1 antibody on one of the four spatially distinct electrodes in each well. XBP-1 was detected with anti-XBP-1 antibody
conjugated with MSD SULFO-TAG.
500000
Company Address
MESO SCALE DISCOVERY®
A division of
Meso Scale Diagnostics, LLC.
1601 Research Boulevard
Rockville, MD 20850-3173 USA
www.mesoscale.com®
400000
300000
200000
100000
0
For Research Use Only.
Not for use in
diagnostic procedures.
Recombinant Human XBP-1 (ng/mL)
Figure 1: Sample data generated with the XBP-1 Kit. Increased signal is observed with the titration of recombinant human XBP-1 protein.

XBP-1 Datasheet
MSD Phosphoprotein Assays
Lysate Titration
Data for recombinant XBP-1 protein using the XBP-1 Kit are presented below.
Sample
(ng/well)
0
1.4
4.1
12
37
111
333
1000
XBP-1 Recombinant Protein
Average Signal
StdDev
%CV
150 9 5.7
2571 179 7.0
12 572
3036 24.2
39 175
4388 11.2
110 760
3928 3.5
239 750
11 727
4.9
369 889
11 688
3.2
495 002
11 299
2.3
For a complete list of products, please visit our website at www.mesoscale.com.
The MSD Advantage
Multiplexing: Multiple analytes can be measured in one well using typical sample volumes of 25 µL or less without compromising speed or performance
Large dynamic range: Linear range of up to five logs enables the measurement of native levels of biomarkers in normal and diseased samples without multiple
dilutions
Minimal background: The stimulation mechanism (electricity) is decoupled from the response (light signal), minimizing matrix interference
Simple protocols: Only labels bound near the electrode surface are excited, enabling assays with fewer washes
Flexibility: Labels are stable, non-radioactive, and conveniently conjugated to biological molecules
References
1. Kopito RR. Aggresomes, inclusion bodies and protein aggregation. Trends Cell Biol. 2000 Dec;10(12):524-30.
2. Kaufman RJ. Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev. 1999 May 15;13(10):1211-33.
3. Mori K. Tripartite management of unfolded proteins in the endoplasmic reticulum. Cell. 2000 May 26;101(5):451-4.
4. Harding HP, et al. Transcriptional and translational control in the Mammalian unfolded protein response. Annu Rev Cell Dev Biol. 2002;18:575-99.
5. Yoshida H, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001 Dec 28;107(7):881-91.
6. Lee AH, et al. XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response. Mol Cell Biol. 2003 Nov;23(21):7448-59.
MESO SCALE DISCOVERY, MESO SCALE DIAGNOSTICS, DISCOVERY WORKBENCH, MULTI-ARRAY, MULTI-SPOT, QUICKPLEX, SECTOR PR, SECTOR, SECTOR HTS, SULFO-TAG,
www.mesoscale.com, SMALL SPOT (design), 96 WELL 1, 4, 7, & 10-SPOT (designs), 384 WELL 1 & 4-SPOT (designs), MSD, MSD (design), and V-PLEX, STREPTAVIDIN GOLD,
SPOT THE DIFFERENCE are trademarks and/or service marks of Meso Scale Diagnostics, LLC.
© 2013 Meso Scale Diagnostics, LLC. All rights reserved.
MK-DS-175-v1-2013Mar
For Research Use Only. Not for use in diagnostic procedures.


Features Datasheet pdf MSD® XBP-1 Kit For quantitative determ ination in human, mouse, and rat whole cell lysate samples Alzheimer’s Dise ase BioProcess Cardiac Cell Signaling C linical Immunology Cytokines Growth Fac tors Hypoxia Immunogenicity Inflammatio n Metabolic Oncology Toxicology Vascula r Catalog Numbers XBP-1 Kit Kit Size Catalog # 1 plate K150QKD-1 5 plate s K150QKD-2 25 plates K150QKD-4 1. XB P-1 2. BSA blocked 3. BSA blocked 4. BS A blocked X-box binding protein 1 (XBP- 1) is a basic-region, leucine zipper ty pe transcription factor that belongs to the CREB/ATF family and mediates one o f the three arms of unfolded protein re sponse (UPR) adaptation to endoplasmic reticulum (ER) stressors. The accumulat ion of unfolded or misfolded proteins i n the ER is a threat to cell survival a nd results in a condition known as ER-s tress.1,2 To overcome this stress, the ER initiates specific signaling pathway s encompassed by the ER stress response .3 Among these are translational attenuation, upregulation of ER c.
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